nickel magnetic beads for his 6 tag protein purification (Bimake Inc)
Structured Review

Nickel Magnetic Beads For His 6 Tag Protein Purification, supplied by Bimake Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nickel+magnetic+beads+for+his+6+tag+protein+purification/nickel+magnetic+beads+for+his+tag+protein+purification/pmc06479270-48-6-16
Average 90 stars, based on 1 article reviews
Images
1) Product Images from "Design of a chromogenic substrate for elastase based on split GFP system—Proof of concept for colour switch sensors"
Article Title: Design of a chromogenic substrate for elastase based on split GFP system—Proof of concept for colour switch sensors
Journal: Biotechnology Reports
doi: 10.1016/j.btre.2019.e00324
Figure Legend Snippet: Colour of the UM, UM10 and E11 proteins. Bacterial pellets after expression under optimized laboratorial set-up and in solution after purification at 1.5 mg/mL for UM and UM10, and at 0.5 mg/mL for E11.
Techniques Used: Expressing, Purification
Related Articles
Magnetic Beads:Article Title: Functional Analysis and Antivirulence Properties of a New Depolymerase from a Myovirus That Infects Acinetobacter baumannii Capsule K45 Article Snippet: The protein was purified by immobilized-metal affinity chromatography (Thermo Scientific) and incubated with a TEV protease overnight at 4°C in a protease/protein ratio of 1/100 (vol/vol) to cleave the SlyD leader protein. .. The protein was repurified using Article Title: Design of a chromogenic substrate for elastase based on split GFP system—Proof of concept for colour switch sensors Article Snippet: Isopropyl-β-D-thiogalactopyranoside (IPTG) inductor was purchased from Acros Organics (Geel, Belgium). .. Article Title: Functional Analysis and Antivirulence Properties of a New Depolymerase from a Myovirus That Infects Acinetobacter baumannii Capsule K45 Article Snippet: 140 The protein was purified by immobilized metal affinity chromatography (Thermo Scientific) and 141 incubated with a TEV protease overnight at 4 °C in a protease/protein ratio of 1/100 (v/v) to cleave 142 the SlyD leader protein. .. The protein was re-purified using Protein Purification:Article Title: Functional Analysis and Antivirulence Properties of a New Depolymerase from a Myovirus That Infects Acinetobacter baumannii Capsule K45 Article Snippet: The protein was purified by immobilized-metal affinity chromatography (Thermo Scientific) and incubated with a TEV protease overnight at 4°C in a protease/protein ratio of 1/100 (vol/vol) to cleave the SlyD leader protein. .. The protein was repurified using Article Title: Design of a chromogenic substrate for elastase based on split GFP system—Proof of concept for colour switch sensors Article Snippet: Isopropyl-β-D-thiogalactopyranoside (IPTG) inductor was purchased from Acros Organics (Geel, Belgium). .. Article Title: Functional Analysis and Antivirulence Properties of a New Depolymerase from a Myovirus That Infects Acinetobacter baumannii Capsule K45 Article Snippet: 140 The protein was purified by immobilized metal affinity chromatography (Thermo Scientific) and 141 incubated with a TEV protease overnight at 4 °C in a protease/protein ratio of 1/100 (v/v) to cleave 142 the SlyD leader protein. .. The protein was re-purified using |